The functional maturity of grafted human pluripotent stem cell derived-islets (hSC-Islets) evaluated by the glycemic set point during blood glucose normalizing process in diabetic mice

Human pluripotent stem cell (hPSCs) derived-pancreatic islets (hSC-islets) are good candidates for replacement therapy patients with diabetes as substitutes deceased donor-derived islets, because they and have infinite proliferation potential. Grafted hSC-islets ameliorate hyperglycemia in diabetic mice; however, several weeks needed to normalize the hyperglycemia. These data suggest require maturation, but their maturation process vivo is not yet fully understood. In this study, we utilized two kinds of streptozotocin (STZ)-induced model mice by changing administration timing order examine time course effects on maturation. We found no immune-compromised when had been transplanted under kidney capsules advance, STZ was administered 4 after transplantation. Of note, blood glucose levels those were stably maintained 100 mg/dL 10 transplantation; lower than mouse glycemic set point (120–150 mg/dL), suggesting that control human points. confirmed gene expression markers pancreatic beta cells tended upregulate during Periodical histological analysis revealed revascularization observed early 1 week transplantation, reinnervation grafted detected at all, even 15 conclusion, our need least mature, a index evaluating ultimate maturity vivo.